Alamarblue cell viability assay reagent e cell viability. Measuring cytotoxicity or proliferation alamarblue assay protocol. Homogeneous, fluorometric cell viability assay resazurin. The alamarblue reagent is an oxidized form of redox indicator that is blue in color and nonfluorescent. Celltiterblue cell viability assay technical bulletin tb317. Optimized alamarblue assay protocol for drug doseresponse. Drugs with antioxidant properties can interfere with cell. The alamarblue dye in its oxidized form is blue in color and nonfluorescent. For determining the effect of a test agent on cell growth, ensure correct controls are included e. Celltiterblue cell viability assay technical bulletinpdf 933 kb english. Cell viability assays such as cell titer blue and alamar blue rely on the reducing property of viable cells to reduce the reagent dye to a product which gives a fluorescent signal.
When incubated with viable cells, the reagent changes. Celltiterblue cell viability assay promega corporation. We optimized the original protocol of alamarblue assay that usually suggests an. The celltiterblue assay typically can detect fewer than 150 cellswell using a. General method for measuring cytotoxicity or proliferation using alamarblue. The alamarblue assay is designed to measure quantitatively the proliferation of various human. Choosing the right cellbased assay for your research. Fluorescence excitation and emission spectra for resorufin. A flow diagram summarizing the celltiterblue assay protocol is shown in figure 3. No qc protocol is recommended for fluorescence since. Celltiterblue cell viability assay protocol promega corporation.
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